The tiny fetal mouse brains developed cortical folds, the authors report today in Cell.
Together, our data provide new insight into the p53 transcriptional network in the developing mouse brain as well as in some of the transcriptional changes that occur during the earliest stages of mouse brain development.
The team's first challenge was to dissect away, at the very start of neural development, the part of the mouse brain that develops into the hypothalamus and then cut tiny slices of this region for use in microarray analysis, a technology that reveals multiple gene activity.
Using mouse brain cells developing in a dish, the group next showed that increasing the levels of the co-activator PGC-1α boosts FNDC5 production, which in turn drives BDNF genes to produce more of the vital neuron-forming BDNF protein.
This study demonstrated that the xenografted tumor in mouse brain parenchyma develops in a very similar manner to those found in patients affected by GBM and can be used to better understand the biology of GBM as well as testing potential therapies.
Light microscopy analysis of paraffin sections of mouse brains with developed glioma detected pathological changes restricted to large hemispheres.
Together, these analyses show that the Vglut2 gene is expressed from midgestation and onwards in the developing mouse brain, including the developing VTA area where it is found in both TH-expressing and non- TH-expressing cells.
A simple and fast HPLC method based on an isocratic, reversed-phased ion-pair with amperometric end-point detection for simultaneous measurement of noradrenergic (MHPG/NA and A), dopaminergic (DOPAC, HVA/DA) and serotonergic (5-HIAA/5-HT) compounds in mouse brain tissue was developed.
Acquisition of light microscopic data at 1-micrometer resolution for an entire mouse brain has been developed.
Besides antibodies, FUS-MB may be used to deliver a variety of imaging and therapeutic molecules to the transgenic mouse brain, including newly developed amyloid-targeted MR probes [26], [27] and siRNA [28], [29].
