Because sAC proteins in mouse brain derive from this alternate promoter, downstream from the deleted exons and inserted IRES-lacZ cassette, one would not expect to find LacZ expression in brain nor a neuronal defect in Sacytm1Lex/Sacytm1Lex mice.
To study whether up-regulation of miR-101 by delivering lentiviral vector encoding miR-101 to the tumor site (post tumor-forming) could sensitize the tumor to radiation, we chose the mouse brain xenografts derived from U87MGD cells because brain tumor treatment depends more on the radiotherapy than other organ tumors due to the brain-blood barrier.
Mouse brain-derived, purified inactivated Japanese encephalitis vaccine (MB JEV) has been locally produced and introduced into Thai National Immunization Program NIPP) since 1990.
Two Tet promoters are compared here, the original PhCMV*-1 and the second-generation Ptight, with respect to the inducibility of the gene of interest in neuroendocrine and neural cells genetically engineered to express rtTA, namely PC12-Tet-On cells and MB-G-18 cells (mouse brain-derived cells with the phenotype of neuron-restricted precursors).
There are 4 main types of JE vaccines currently in use: inactivated mouse brain-derived vaccines, inactivated Vero cell-derived vaccines, live attenuated vaccines, and live recombinant vaccines – all have been licensed and WHO-prequalified.
Transplantation of mouse brain-derived NPs into the CNS of rodents with experimental autoimmune encephalomyelitis (EAE), the animal model of MS, attenuated the brain inflammatory process and severity of clinical disease [10] [12].
Mouse brain-derived, inactivated JE vaccines (JE-MBs) are available and are internationally accepted.
Over the past half-century, a mouse brain-derived inactivated vaccine has been used internationally for active immunization.
These vaccines have better safety profiles and fewer side effects compared with the mouse brain-derived inactivated vaccine [ 24- 26].
The serum BDNF concentration was measured using the mouse brain-derived neurotropic factor ELISA kit (R&D Systems, USA) following the manufacturer's instruction.
