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The conditionally immortalized mouse brain capillary endothelial cell line (TM-BBB) was performed as previously described [61].
Mouse brain capillary fraction was prepared as described [ 25].
[I]hAβ 1-40) internalization was analyzed using conditionally immortalized mouse brain capillary endothelial cells (TM-BBB4).
CRT is localized in mouse brain capillary endothelial cells and mediates CT influx transport across the BBB [ 21, 64].
The absolute amount of transporter protein in mouse brain capillary endothelial cells was determined by mass spectrometric analysis [ 26].
Immunohistochemical studies have indicated that CRT is localized at both the luminal and abluminal sides of mouse brain capillary endothelial cells in the adult brain [ 21, 64].
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Isolated mouse brain capillaries were prepared as described previously [ 29].
RT-PCR analysis confirmed the expression of VDR mRNA in mouse brain capillaries.
Vitamin D receptor (VDR) mRNA was detected in mouse brain capillaries, suggesting that 1,25(OhasD3 has a VDR-mediated genomic action.
The transporter protein expression levels of Mrp4, Oat3, Oatp1a4 and Pgt in mouse brain capillaries were simultaneously quantified by LC-MS/MS (Table 2).
Similarly, Mrp 2 multidrug resistance associated protein 2 and breast cancer resistance protein (BcRP) and CAR are detected and expressed in rat and mouse brain capillaries [ 170].
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