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Rab3a was cloned from mouse brain cDNA.
The 1022 bp PCR product from mouse brain cDNA was then cloned into a pcDNA3.1/V5-His-TOPO expression vector.
RNF10 has been shown to be ubiquitously expressed and has been previously isolated from a mouse brain cDNA library [13].
Full-length mouse LRRK2 cDNA was cloned by PCR from mouse brain cDNA prepared in our lab.
The full-length β2L coding sequences amplified from monkey and mouse brain cDNA libraries also confirmed the presence of β2L in these two species (Figure S2).
However, a further yeast-two-hybrid screen using mouse eEF1A2 as bait for a mouse brain cDNA library also failed to pull out any eEF1B subunits [67].
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Primers were designed based on intron 4 of GABAB1a and common 3' UTR of known isoforms, and PCRs were performed using the human and mouse brain cDNAs.
The entire insert was sequenced and used as a bait to screen a random-primed Mouse Adult Brain cDNA library constructed into pP6.
We have used a yeast mutant, cog6Δ, which is particularly sensitive to moderate αSyn expression, for screening a mouse brain-specific cDNA library in order to identify mammalian proteins that counteract αSyn toxicity.
The cDNA of mouse ubiquitin (Ub) was amplified from mouse brain and testis cDNA (Clontech-Takara Bio, Shiga, Japan) by PCR with the following pair of oligonucleotides with a BamHI linker (underlined) or a stop codon (bold): Met primer, 5′- GGATCCATGCAGATCTTCGTGAAGACCCT-3′ and stop primer, 5′-ACAGCT TTATTTGACCTTCTTCTTGG-3′.
To determine whether the neuronal form of pp60c-src is encoded by a brain-specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c-src probe and a 3.8-kilobase c-src cDNA clone was isolated.
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