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For the isolation of the crude membrane fraction of mouse brain, brains from the C57BL/6J mice were homogenized in a buffer containing 250 mM sucrose, 3 mM imidazole, 1 mM EDTA and protease inhibitors and were centrifuged at 1,000×g for 10 min. The supernatant was further separated by ultracentrifugation at 100,000×g for 1 h.
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Optimized formulations containing the luciferase reporter gene were delivered to mouse brain by intraventricular brain injections.
"They said putting the mouse brain on line would accelerate brain research throughout the world," Mr. Allen recalled.
Predicted target genes were further evaluated using the online tool AmiGO (http://amigo.geneontology.org/amigo), the Vienna RNA webserver (RNAfold, http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi) and the Allen mouse brain atlas (http://mouse.brain-map.org/).
Given enough of these pieces, it might one day be possible to compile a complete map of a mouse brain and, eventually, the human brain.
They found that 586 genes in the mouse brain were overactive in following brain injury and that 10percentt of these genes were dampened after stem-cell injection.
And it works equally well for mapping the neural circuits in small chunks of the mouse brain, and potentially the human brain.
We describe the use of stimulated Raman scattering (SRS) microscopy for differentiating healthy human and mouse brain tissue from tumor-infiltrated brain based on histoarchitectural and biochemical differences.
This image shows a map of neural activity in the awake mouse brain, top, and pattern of brain communication when the sensory projection neurons are optically driven, bottom.
To illustrate the challenge, he noted the worm c. elegans has 102 neurons in its brain, while the mouse brain is magnitudes larger, at 108 neurons.
We provide practical examples on how to use The Virtual Mouse Brain to simulate brain activity, such as seizure propagation and the switching behavior of the resting state dynamics in health and disease.
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