(A ) Schematic of a dorsal whole mount view of the embryonic mouse brain and (B ) Sagittal section of the mid-hindbrain region.
In vivo T2*-weighted MRI images show a 14-month-old wild-type (A) and transgenic (B) mouse brain.
In (c - d), transverse hippoc - dl sectransverse a few small sparse immunoreactive NTFs in group A (c) thippocampaly contrasectionsthe numeroushowrongly immunoreactive Nfew obsmalld in the corresparseng mouse braimmunoreactiveom group B (D; EB102).
In (g)–(i), transverse panoramic sections of the dentate gyrus show scarce dystrophic astrocytes in group A (g), which contrasts with densely dystrophic reactive astrocytes observed in the corresponding mouse brain sections of groups B (H; EB102) and C (I; PBS).
(d)–(f) show transverse sections of the hippocampal and cortical regions showing a few sparse immunoreactive NFTs in group A (d) with reduced immunoreactivity, which contrasts sharply with the numerous immunoreactive NFTs in the corresponding mouse brain sections of groups B (E; EB102) and C (F; PBS).
Photomicrographs from brain sections from phase I show some immunoreactive B cells in mice from group A (EB101), especially at the hippocampal regions), this leukocyte surface marker being of a lower density in the mouse brains of group B (EB102; Figure 2 k)) and C (PBS; Figure 2(l)).
Additionally, since there was no concomitant overexpression of PDGFRα in tumors generated from RCAS/ tv-a mediated PDGF-B expression, itv-a mediatederesting to determine whether targeted co-overexpression of PDGFRα and PDGF-B in thexpressionait will generate gliomas with similar histopathological characteristics as does PDGF-bealone.
Straightforward immunofluoresence and Western blot assays demonstrated that cultured astrocytes from mouse brain express high levels of CK-B, which corroborates findings with immunostaining and in-situ hybridization on whole mouse brain that appeared in other reports [23], [34].
(B ) Coronal section of the mouse brain showing the location of VMHdm (Allen Brain Atlas).
