Both splice variants of exons 14, 16 and 22a were present in human and mouse brain, at all ages and in all brain regions examined.
Although ABA data may extend from mouse brain tissue, all genes listed in Table 1 are orthologous to rat and human genes according to the Mouse Genome Informatics database (http://www.informatics.jax.org).org
For mouse brain samples, all mice striatum and cortex tissues protein extracts were obtained in the following ice-cold extraction buffer: 50mM Tris.HCl pH 7.4, 250mM NaCl, 5mM EDTA.Na2, 1% Triton-100 with 1× Complete Protease Inhibitor, and then homogenized by electric homogenizer.
While elevated Aβ levels in very young NEP knockout mice were already described [11], [12], we, for the first time, observed significantly higher Aβ levels in NEP-deficient mouse brains at all investigated time points in comparison to their age-matched wild-type mice (Figure 2).
Probes were hybridized to adult mouse brain sections and all showed small relatively equally sized signals throughout the brain (Fig. 4B), except Gtl2 (Fig. 4A).
Ze et al. [68] treated mice with three doses of TiO2 NPs nasally for 90 days and found that the levels of superoxide (O2 −), H2O2, MDA, protein carbonyls, and 8-hydroxy-2′-deoxyguanosine in the mouse brain were increased in all groups compared with control animals.
An 11.7 T 89 mm vertical bore Bruker BioSpin Avance DRX500 scanner (Bruker BioSpin Inc, Billerica, MA) equipped with a Micro2.5 gradient system was used to acquire all mouse brain images and spectroscopic data with a 35 mm linear birdcage radio frequency (RF) coil.
t tests with Welch's correction for unequal variances were performed for all mouse brain and liver phosphopeptide biological replicates.
According to the Allen brain atlas, Cryaa, Sik1, and Rrp1b are expressed in the adult mouse brain, including the hippocampus (all three), the cortex (Sik1 and Rrp1b), and the cerebellum (Rrp1b).
For VOI analysis, processed images were subsequently co-registered with a predefined mouse brain VOI template (encompassing all relevant brain regions) aligned with the aforementioned Mirrione CT/MRI atlas and tracer uptake values were extracted for each delineated VOI.
