The developmental expression pattern of OPN has not been previously investigated and, therefore, our result demonstrating temporally limited overexpression in mouse bone is interesting (Fig. 3).
This method enables us to analyze precisely comparable sites of the loaded and contra-lateral control tibiae because the effects of loading are site-specific and the mouse bone is small.
In a more recent study by Liu et al. using the same cell line, metastasis to both human and mouse bone was detected by whole-body BLI, which contradicted the group's earlier result (Kuperwasser et al., 2005; Liu et al., 2009).
The mouse bones were carefully oriented for longitudinal sectioning to include observing the epiphyseal plate as well as the distal region of the bone in the same section (Fig. 3A, top panel).
Gross abnormalities of KO mouse bones were not observed as shown in the X-ray of bone from both FL and KO mice (Figure 7), suggesting that even OGR1 might even have a role in osteoclastogenesis, the overall effects are not apparent and that a redundant role of other genes may be involved in vivo.
For analysis of bone marrow endothelium, mouse bones were harvested 4 d post-irradiation, crushed and then stained with a panel of haematopoietic/endothelial markers exactly as described previously by Hooper et al (2009).
On the other hand, the reported crystal size from fish, bovine, chicken, and mouse bones was similar, about 40 nm in length and 30 nm in width, with different distribution in each animal studied [ 8].
In addition, our preliminary data using adult mice bones are also presented in this table, and demonstrates positional identity marker expression, at least, appears to be conserved between these two species).
BMD and bone mineral content (BMC) of mouse femoral bone were determined with a PIXImus mouse densitometer (GE Lunar, Waukesha, WI, USA).
Human osteoclast precursors, isolated from mouse bone marrow, were differentiated into TRAP+ multinucleated cells, which were larger in size and number in the S100A7-control breast cancer cells as compared to the S100A7-downregulated cells in transwell assays.
