Figure 2 provides an example of different imaging acquisitions of various mouse bones used for skeleton phenotypes and bone repair studies.
However, all the earlier studies on the Brtl/+ mice bones used 2- [ 8– 18] 18] and sometimes both 2- and 6-month-old animals [ 8, 10], and the FTIRI and micro-CT data for the teeth were limited to jaws from these earlier studies.
The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma.
Under isofluorane (Escain, Mylan-Japan, Tokyo, Japan) anesthesia and aseptic conditions, a platform for head fixation was made on the mouse cranial bone using synthetic resin (Superbond C &B, Sun Medical, Tokyo, Japan) and one 15-mm stainless bolt.
Neutrophils were then prepared from mouse bone marrow using the EasySep Neutrophil Enrichment Kit (Stemcell Technologies).
MPP and CDP were obtained from mouse bone marrow, using in vitro culture with a specific cytokine cocktail and FACS sorting [ 12, 13].
This study examined the role of NPP1 in osteocytes, osteoclasts and cortical bone, using a mouse model lacking NPP1 (Enpp1 −/−).
Our objective was to study the influence of active PAI-1 on geometric, biomechanical, and mineral characteristics of bone using transgenic mice that over-express a variant of human PAI-1 that exhibits enhanced functional stability.
Mouse BMCs were flushed from femur bone using α-minimum essential medium eagle (α-MEM).
In this report, we have determined the in vivo effect of ERRα on bone, using knock-out mice.
