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In terms of a possible thermal effect on body, we confirmed that exposure to RF-EMF in our system could not affect mouse body temperature as shown in Fig. S2.
In this review, we briefly summarize applications of CAMM in immunoassay development, including assisting in hapten design, explaining cross-reactivity, modeling antibody antigen interactions, and providing insights into the effects of the mouse body temperature on the three-dimensional conformation of a hapten during antibody production.
Mouse body temperature was monitored by fiber optic probe.
Mouse body temperature was monitored with a rectal probe and thermistor (THM100; Indus Industries, Houston, TX) and maintained at 37±2°C with a temperature-controlled heating pad (THM100).
While under anesthesia (xylazine 10 mg/kg i.p; ketamine 150 mg/kg i.p). the mouse body temperature was monitored with a rectal probe and maintained at 38°C using a homeothermic blanket.
All of the anesthetized mice were initially anesthetized in their home cage, and, once sedated, they were transferred to a Thermocare® ICS therapy warmer unit (Thermocare, Incline Village, NV) either set to maintain a mouse body temperature of ∼37°C or with the heating element turned off, the latter setting used to achieve propofol-induced hypothermia.
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Mouse body temperatures was monitored closely and maintained between 34°C and 36°C using infrared heating.
During sedation and anesthesia, it is imperative to carefully monitor and support mice body temperature, heart and respiratory rates, mucous membranes, and the degree of CNS depression.
Throughout surgery and subsequent physiological experiments, the mouse's body temperature was maintained at 37°C via a feedback-controlled heating pad.
Mouse core body temperature was constantly monitored with a rectal probe and kept at 37°C with a heating pad.
The mouse's body temperature was maintained to reduce vasoconstriction by keeping it on a heating pad with its core temperature monitored rectally.
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