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The tumor volumes and mouse body size were measured once every 4 days.
Depending on mouse body size, smaller mice were raised within the cassette by fashioning rectangular bedding made from gauze or folded paper towel.
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Further subdividing the data by the body weight of each animal revealed that much of the remaining variability in each parameter could be accounted for by the mouse's body size.
The deletion of Irs1 in mice reduces body size (Araki et al. 1994) and increases lifespan (Selman et al. 2008), whereas complete disruption of Irs2 causes diabetes due to insulin resistance and pancreatic β cell failure (Withers et al. 1999; Burks et al. 2000).
Whereas Ing1−/− mice had reduced body size [52], Ing2−/− mice grew normally.
However, if the complete system is to be fully implanted and the subject allowed to freely move inside a cage, the mouse's small body size sets harsh constrains on the size and power consumption of the required electronics.
Heterozygotes (Stx-t+/−) and wild-type mice are indistinguishable, but STX-T-deficiency mice show smaller body size (Fig. 1A) and dramatically decreased body weights compared with the weights of wild-type mice at both embryonic days (E) 18.5 and postnatal day (P) 0 (Fig. 1B).
Importantly, a 70% decrease in the level of MDM2 is sufficient for survival but results in multiple overt p53-dependent phenotypes in Mdm2puro/null mice, including reduced body size and lymphopenia that are not manifest in mice with a more modest decrease in MDM2 [38].
Similar to RasGrf1 null mice generated in a different strain [ 8], our knockout mice display reduced body size.
Thus, although stride parameters of pcd mice were different overall from controls, they were comparable to those predicted for control mice of similar body size walking at similar speeds.
Mmp13−/− mice have normal body size.
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