The in vitro stability was assessed by incubating 6 μCi [131I]-PARPi in mouse blood for 0 to 60 min at 37 °C.
Enzyme linked immunosorbent assays (ELISA) were used to screen deer mouse blood for IgG antibodies to SNV.
Colonized mosquitoes were maintained on mouse blood (for egg laying) and given 10% sucrose ad libitum.
Serum samples were prepared from mouse blood for analysis of the total serum IgG1 and IgE levels by ELISA Bethyl Laboratoriess, Montgomery, TX, USA) according to manufacturer's instructions.
The spirochetes observed in the squirrel's blood failed to grow in BSK-H medium after passage in the laboratory mouse; however, we acquired DNA sequences from infected squirrel and mouse blood from PCR amplicons for 6 spirochete loci including 16S rDNA, flaB, gyrB, glpQ, IGS, and vtp.
The number of exflagellation centers in mouse blood was significantly higher for puf2 parasites than for WT parasites (Figure 3C), suggesting that male gametocytes contribute to the increased gametocytogenesis in Pbpuf2 parasites, in full support of the data reported for P. falciparum Puf2-deficient parasites [22].
Benzene showed higher affinities for RBCs in the deoxygenated HbS, rat, and mouse blood and higher affinity for plasma in the guinea pig blood.
Hemoglobin concentration was determined using an Animal Blood Counter (SCIL, Viernheim, Germany), which had been calibrated for mouse blood within 2 hours after blood taking.
To investigate possible side-effects, COHb levels in cardiac puncture blood were determined in LPS-challenged animals, using a hemoximeter with inbuilt algorithms for mouse blood (OSM3, Radiometer, Crawley, UK).
After copy number correction, the total SNCA concentration in the mouse blood showed a trend for the reduction in the PD risk-associated Rep1-261 bp animals, but the variability, in particular within the Rep1-259 bp genotype, was large (Fig. 7B).
PCs for rat and mouse blood were about 100% higher than those for human and guinea pig blood, but the PC for deoxygenated HbS blood was only slightly higher than that for HbA or oxygenated HbS blood.
