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She and her colleagues had demonstrated a surprisingly simple way of turning ordinary body cells – she used mouse blood cells – into something very much like embryonic stem cells.
Mice and people are so similar at the cellular level that the chemical signals released by the mouse blood cells were presumably recognized by the human embryonic stem cells and taken as a cue to morph into the blood-forming cells of the bone marrow.
Simply giving mouse blood cells an acid bath is enough to produce so-called pluripotent cells that can develop into any cell type in the body, they report this week.
Lee conducted a real-time attempt to reproduce a controversial experiment published in Nature in which researchers claimed to have turned adult mouse blood cells into pluripotent stem cells.
Simply giving mouse blood cells an acid bath is enough to produce so-called pluripotent cells that can develop into any cell type in the body, they report in two new papers this week.
We showed that the radionuclide Sr, when injected into mice was rapidly localized to skeleton, and led to a dramatic effect on gene expression in mouse blood cells.
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The determined IC50 was approximately 3 μ M for human and mouse blood cell SOD1.
A recent study found that mouse blood cell-derived iPSCs more closely resemble ESCs epigenetically and functionally than those derived from fibroblasts.
Smears or cytospin specimens of mouse blood cell samples were stained by a modified Wright Giemsa staining method, using Diff-Quik kit (Sysmex, Kobe, Japan).
The mice blood cells were washed three times in stroke-physiological saline solution (0.9% NaCl) and centrifuged at 4 °C, 2000 rpm for 5 min.
In the mouse blood derived cells, the majority of cells were CD45+ collagen I+.
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