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The enhanced survival ability seemed to have a significant influence on whether hPSCs can integrate with the ICM of mouse blastocysts during in vitro culture.
The global gene expression in mouse blastocysts during delayed implantation and activation was also reported [4].
However, in vitro exposure of curcumin to mouse blastocysts during the early postimplantation stages had adverse effects in a dose-dependent manner [ 164].
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Can scientists take embryonic stem cells from mouse blastocysts, for example, and use them to cure diseases in mice?
Kunath, T. et al. Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts.
James, D., Noggle, S. A., Swigut, T. & Brivanlou, A. H. Contribution of human embryonic stem cells to mouse blastocysts.
After being labeled with a Green Fluorescent Protein (GFP) marker, T (5:7) mESCs were injected into B6D2F1 (C57BL/6 and DBA/2 hybrid) mouse blastocysts (Fig. 4a), and the ESC-injected blastocysts were transferred to pseudo-pregnant female C57BL/6 mice.
Tachi, C. Partial characterization of macromolecular components in fetal bovine serum required for development of mouse blastocysts cultured in vitro.
To assess the effect of equilibration time on the DNA integrity of vitrified-warmed mouse blastocysts.
(C) Survival curve of hESC in mouse blastocysts over time.
Bottom 2 rows, E3.5 and E4.5 mouse blastocysts with iNANOG cells (GFP); scale bar, 100 μm.
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