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The morphogenesis of the mouse blastocyst provides an excellent system to study these two mechanisms.
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Solter, D. & Knowles, B.B. Immunosurgery of mouse blastocyst.
Strumpf, D. et al. Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst.
Rayon, T. et al. Notch and hippo converge on Cdx2 to specify the trophectoderm lineage in the mouse blastocyst.
We also performed live imaging to monitor the behavior of iNANOG cells in the mouse blastocyst.
Mouse blastocyst implantation occurs at ca. 3.5 gd [9].
Under these conditions, CD98 silencing significantly impaired mouse blastocyst adhesion (Figure 5D).
Gata6 regulates PE genes [41] antagonising Nanog within the mouse blastocyst [42].
Trophoblast stem (TS) cells were derived from the mouse blastocyst by cultivation in medium containing FGF4 [4].
Several types of proliferating cells have been derived from the mouse blastocyst.
Endometrial overexpression of CD98 or tetraspanin CD9 greatly enhanced mouse blastocyst adhesion, while their siRNA-mediated depletion reduced the blastocyst adhesion rate.
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