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In the mouse bioassay, when the injected dose is high, mice typically develop signs of botulism within 8 hours.
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The results confirm that no cell substrate propagated infectivity detectable by mouse bioassay, even when mice were observed for their expected lifespan.
In this study we investigated the ability of wild type mouse bioassay to identify BSE when it is present in an excess of scrapie during primary isolation using histopathology, immunohistochemistry (IHC) and Western blot.
When substituting the mouse bioassay for lipophilic marine algal toxins in shellfish with analytical methods, science based factors of relative toxicity for all analogues that contribute to health risk to consumers are necessary.
The GT-13A binder showed a high degree of segregation between non-hydroxylated and hydroxylated toxins indicating that when samples are analysed and compared with the mouse bioassay using this binder the toxin content of a sample would be underestimated if the toxin composition is predominately the hydroxylated toxins, NEO or GTX1/4 (Campbell et al. 2007).
When performed, diagnostic testing on food or human specimens was conducted at NCDC by using the standard mouse bioassay for detecting botulinum toxin (9 ).
The oysters are tested by government scientists using the "test du souris", or the mouse bioassay test.
Routine monitoring of these toxins relies on the mouse bioassay.
This assay is rapid and straightforward and evades ethical issues associated with mouse bioassay.
The current reference method for the analysis of these toxins is the mouse bioassay (MBA).
GP-diluent is used in the mouse bioassay for BoNT detection.
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