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The mouse bioassay represents the gold standard method for investigation of the biologic properties of prions and the discrimination of TSE strains.
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While mouse genotype may have played a role in the outcome, it is also probable that cervid PrP transgenic mouse bioassay simply represents a more sensitive detection system for prions in excreta.
This bioassay represents the adaptation of the established stickleback androgen bioassay in which the androgen specific biomarker spiggin is measured using an ELISA (Katsiadaki et al. 2002a).
For example, if a bioassay represents a receptor that belongs to a toxicity pathway relevant to the target animal toxicity, then this bioassay should provide useful information, such as receptor/pathway perturbation.
It is highly unlikely that the official reference methods currently prescribed in legislation, the rat bioassay and the mouse bioassay, can be adapted to the proposed levels [ 17].
The mouse bioassay can also be used to differentiate BoNT serotypes (4 ).
Only one of them was determined to be toxic in mouse bioassay.
Also, the Endopep-MS method needs to be validated against the mouse bioassay.
As receptor binders for PSP toxins reflect toxicity in a similar manner to the mouse bioassay, it was envisaged that they could be an ideal candidate for the development of an SPR assay to replace the mouse bioassay.
Lot-to-lot variability of the toxin as well as individual variation between the mice is known to confound the mouse bioassay; previous work has shown the mouse bioassay to be sensitive to approximately 20 pg/mL.
The oysters are tested by government scientists using the "test du souris", or the mouse bioassay test.
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