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Sections were scaled to account for shrinkage using the distance between electrode tracks and aligned with the corresponding mouse atlas sections [9] to estimate the stereotaxic coordinates of each recording site.
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Briefly, 7µm serial sections between bregma −0.82 and bregma −1.8 (according to mouse atlas by Paxinos and Franklin, [17] were analysed. Sections were stained for myelin with Luxol-fast-blue periodic acid-Schiff base (LFB-PAS) (Sigma-Aldrich Inc., St . Louis MO).
At alternate sections, the boundaries of the hippocampus were determined using the stereotaxic mouse atlas [20].
For BrdU+ cell counting, brain coronal sections including DG (dentate gyrus) were selected for comparison using a mouse atlas.
Coronal sections at brain levels (+1.54 mm, −1.70 mm, and −5.88 mm re: bregma, Franklin & Paxinos mouse atlas) were selected for mass spectrometric imaging (MSI), as they contain regions that are known to be affected by chronic alcohol consumption.
The tissue sections were selected between −1.46 mm and −2.46 mm posterior to the bregma in reference to the mouse atlas for each animal [ 20].
The co-ordinates of the clusters were reported according to the conventional Lambda-Bregma system in the Allen Mouse Atlas (http://mouse.brain-map.org/atlas/index.html).html
Seriation was conducted on simulated, retinal or Mouse Atlas SAGE data using the custom MATLAB implementation.
MAPaint software, developed by the Edinburgh Mouse Atlas Project [32] [34], was used to analyze these vacuoles.
Another example of a mouse atlas that provides gene expression data for the mouse brain is the GENSAT brain atlas.
The Edinburgh Mouse Atlas Project offers no TEM images (http://www.emouseatlas.org/emap/home.html).html
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