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Briefly, Betaig-h3- or empty vector-transfected tumour cells were injected subcutaneously into nude mice at the left flanks.
To prove it, Huh7 cells with or without MSCs were subcutaneously injected into NOD/SCID mice at the left or right side of the same mouse.
Mouse on the left was a control.
Place your cursor over the word, at the end of the "d", then hold your left button down and move the mouse to the left.
Hepa 1 6 tumor bearing mice were established as following: mice were injected subcutaneously at the left flank with 3 × 10 Hepa 1 6 cells in 200 μL RPMI 1640 (Biowest) or 200 μL RPMI 1640 alone as control.
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Radiolabelled tracer ([68Ga]AAZTA-MG ~ 5 MBq) was administered retroorbitally into tumour xenograft-bearing nude mice with a positive tumour at the left flank (2*106 CCK2+ cells) and a negative tumour at the right flank (2*106 CCK2− cells).
Clicking on the mouse protein identifier ENSMUSP00000108893, then on the Gene ontology link at the left shows the GO terms associated to the mouse protein.
We engrafted 2 × 10 U87 cells at the left and right flanks of the mice.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com