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Mouse primary cerebellar granule neurons (CGNs) were isolated from 10 days old mouse as previously described (Xie et al., 2012).
Dermal thickness was analyzed with a Nikon Eclipse 80i microscope (Nikon, Badhoevedorp, The Netherlands) by measuring the maximal distance between the epidermal-dermal junction and the dermal-subcutaneous fat junction at 4 different skin sections in each mouse, as previously described (Yoshizaki et al., 2010).
injection of ketamine (100 mg/kg) and xylazine (6 mg/kg), and then 2×105 MDA-MB-231-Luc2 cellsuspendeded in 100 µl of PBS were injected into the left ventricle of each mouse as previously described [26].
Fpg-sensitive sites detection was performed in 1x10 bone marrow cells per mouse as previously described [ 68].
Detection of mitochondrial potential was performed using JC1 staining kit on neurons derived from sciatic nerves isolated from adult mouse as previously described (Brunetti et al., 2012).
> -wrap-foot> A set of genes was selected to represent the transcriptional signature of MaSC cells in mouse, as previously described (Lim et al., 2010).
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Cdc42 cardiomyocyte knockout mice were generated by crossing Cdc42/flox mice with MLC-2a Cre mice, as previously reported [22].
Islets were isolated from 10- to 16-week-old mice as previously described (Rorsman and Trube 1986).
T cells were isolated from the spleens of female NMRI mice as previously described (Baumer et al. 2010).
Intraperitoneal injection of 1 mg of mtDAMPs was used as experimental model in wild type and RAGE KO mice, as previously described [1].
We generated patDp/+ mice as previously described [13].
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