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To prepare the bladder for tumour implantation, a point lesion was induced in the bladder wall by an electrocautery unit (Micromed MD1; level 3; 2 pulses per mouse) as already described (Dobek & Godbey, 2011).
However, RT-qPCR analysis showed a two-fold increase in PARK2 expression in liver and WAT of obese B6.V-Lepob/J mice compared with wild-type mice, revealing that its expression is positively correlated with body fat mass in mouse, as already observed in chicken.
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Finally, TDP-43 was not found among the aggregated proteins in the G93A SOD1 mice, as already reported in another study [49].
Th2-biased mouse strains showed increases in airway hyper-reactivity compared to Th1-biased mice, as already shown by different other research groups in other asthma models [1], [17] [20].
We found enhanced levels of Akt in both TA and diaphragm muscles from mdx mice as already described.
Indeed, the amount of C. albicans in lungs cannot be substantially sustained over time in immunocompetent BALB/c mice, as already described elsewhere [ 32].
We note that these structures are strongly labeled by both Sho peptide directed antisera in wt mice, and are distorted and attenuated in prion-infected mice (not shown), as already described by others (Hogan et al, 1987; Johnston et al, 1997; Brown et al, 2001).
DNA extraction of tail clips samples from hybrid mice was performed as already described [ 20].
Cerebellar Granule Cells were derived from cerebellum of P7 C57Bl/6 mice and generated as already published [ 95].
Spleens from VRP- neu-immunized and VRP-HA-immunized mice were prepared as already described for tetramer analysis.
For human and mice, as MspI already provides good coverage of core CpG islands, a non CG containing enzyme (ApeKI, recognition site G^CWGC) was used in combination with MspI to improve coverage in CpG poor regions.
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