A mouse tissue array containing cores of cortical tissue from wild-type C57BL/6J mice at a variety of prenatal, perinatal, and postnatal timepoints, was immunofluorescently stained for MeCP2 and EGR2 protein and fluorescence was quantified to determine the developmental ontogeny of MeCP2 and EGR2 in mouse cortex.
Poly A+ RNA purified from both AG and PG PMEFs were subjected to RNA expression profiling using an Incyte mouse GEM1 array, containing 8,638 cDNA probes corresponding to identified genes [47].
Gene expression was assessed using the 4 × 44 K Mouse Agilent array containing approximately 44,000 oligonucleotide probes, representing 34,204 annotated genes including approximately 21,000 unique genes.
Finally, increased Spalax blood vessel density (Avivi et al. 2005) and differential expression profiling, using a mouse cDNA array containing 15,000 gene elements, uncover dramatic species-specific responses to hypoxic stress among numerous genes involved in angiogenesis, apoptosis, and oxidative stress (Avivi et al. 2006).
Thymic gene expression of 15 week old Hexb+/−FcRγ+/+ and Hexb−/−FcRγ+/+ mice were examined with Affymetrix® Mouse Genome 430 2.0 Array containing 45101unique mouse gene sequences.
An aliquot of 15 μg of RNA per sample was then hybridized to a Affymetrix gene chip, the mouse Genome 430 2.0 array, containing 45,101 probesets and variants from over 34,000 well-characterized mouse genes, using a standard protocol suggested by the Affymetrix menu.
Messenger RNA expression profiling was performed using the Affymetrix GeneChip Mouse Genome 430 2.0 Array containing probes to query more than 45,000 transcripts.
Labeled cRNA was fragmented and hybridized to the Affymetrix Mouse Genome 430 2.0 Array (containing ~ 34,000 genes), as described in the Affymetrix GeneChip protocol.
Total whole lung RNA was used after passing quality control using an Agilent Bioanalyzer 2100 (Agilent Technologies Inc .. GeneChip analysis (Affymetrix) on the Affymetrix Mouse Genome 430 2.0 array containing > 39,000 transcripts was performed on each treatment group in triplicate.
Based on the data presented here, we propose that mouse rDNA array contains several variant types.
