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Detailed tracing studies are necessary to further establish whether or not the cytoarchitectonically defined frontal mouse areas are all prefrontal areas indeed.
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In mouse cells these areas are generally enriched in the H3K9me3 histone PTM [8], [51].
They applied immunohistochemical and biochemical approaches to analyze cellular and molecular changes indicative of cell death in the hippocampus and frontal cortex of the mouse brain; these areas are thought to be crucial in mood control.
Place the box on a level surface where you know the mouse runs (these areas are almost always alongside walls and are easily identified by droppings).
For each mouse, adipocytes areas were determined in at least three histologic sections and 300 total adipocytes.
The cytoarchitectonic definition of mouse prefrontal areas is essential for stereological studies on the total number of neurons and/or glia cells in the distinct cortical areas using Nissl stainings (e.g., Rajkowska et al. 2004).
No lesions were detected in aortas of C57Bl/6J mice, whereas in LDLR-/-ApoB100/100 and IGF-II/LDLR-/-ApoB100/100 mice lesion areas were 61.1 ± 8.7% and 60.0 ± 7.9% of the total aortic area, respectively.
However, in CARD9−/− mice, the fibrotic areas were significantly smaller than in WT mice with Ang II treatment.
In XIAP-KO mice, the same areas were affected as in the wild-type mice.
In control mice, no plaque areas were seen and ROIs were defined for healthy vessel wall, adventitia, and muscle.
Impaired wall motion with hypokinesia was common in LDLR-/-ApoB100/100 (in 10/13 mice, 76.9%) and IGF-II/LDLR-/-ApoB100/100 mice (in 8/11 mice, 72.7%), and akinetic areas were observed in altogether four animals: one LDLR-/-ApoB100/100 and three IGF-II/LDLR-/-ApoB100/100 mice.
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