Sentence examples for mouse aorta was from inspiring English sources

For the quantification of the aortic plaques, the mouse aorta was carefully harvested and stained with Sudan IV (Sigma, Germany).

RAW-Blue cells were labeled with the acetomethoxy derivative of calcein (1 μM), and adhesion to the mouse aorta was carried out as described previously [ 20, 21].

The present study showed that the main determinant of depolarization-induced contractions of the mouse aorta was the influx of extracellular Ca2+ via L-type Ca2+ channels.

To further confirm PXR-dependent regulation of CYPs in the vasculature, in vivo CYP induction in the PXR wild-type and knockout mouse aorta was examined 24 h after ip PCN administration.

Recently, we showed that the relaxing efficacy of NO in mouse aorta was dependent on the contractile agonist, and more specifically, decreased when the contraction was mainly elicited via L-type Ca2+ influx as with elevated extracellular K+, but increased when Ca2+ influx was partially inhibited with L-type Ca2+ channel blockers [ 30].

Sterols in the mouse aorta were analyzed by gas chromatography and mass spectrometry (GC/MS) as previously described [47].

Endothelial cells from mouse aorta were grown at low density in collagen-coated chambers in experimental and control co-culture RPE supernatants for 3 days.

BH4, BH2, and biopterin (B) levels in tissue homogenates or intact mouse aorta were determined by HPLC followed by electrochemical and fluorescence detection, as previously described [16].

Photographs were taken using a fluorescence macroscope (Carl Zeiss, Thornwood, NY, USA), and the number of macrophages that adhered to the mouse aorta were counted using the MetaMorph imaging analysis system (Molecular Devices) and normalized to the total aortic area.

Ring-shaped explants of mouse aorta were then embedded in a rat tail interstitial collagen gel (1.5 mg/ml) (15) prepared by mixing 7.5 volumes of 2 mg/ml collagen (Collagen R, Serva, Heidelberg, Germany), 1 volume of 10 x MEM, 1.5 volume of NaHCO3 (15.6mg/ml) and approximately 0.1 volume of 1M NaOH to adjust the pH to 7.4.

Compared to wild-type mice, ROS production in diabetic OVE26 mouse aortas was significantly increased.