Antiserum generated in mice immunized with the Ap97 vaccine was used to test the ability of the chimeric protein to induce the production of antibodies specific for each of its component subunits, ApxN and P97C, as well as to the whole chimeric protein.
Antiserum generated against rat α-lactalbumin was generously provided by Dr Kurt E Ebner at the University of Kansas Medical Center.
The same peptide was also recognized by the antiserum generated in our laboratory.
In contrast to the NIBSC antiserum, more well defined rings for rHA were achieved using antiserum generated against recombinant antigens.
Sheep anti-DOβ antiserum generated against recombinant full-length human DOβ.
These authors used an antiserum generated in a species other than that used to raise the RIA's primary antiserum (rabbit anti-bPL).
The polyclonal anti-GFAP antibody is a purified immunoglobulin fraction of rabbit antiserum generated to bovine spinal cord GFAP.
TAP protein was determined by Western blot using a goat anti-mouse TAP1 polyclonal Ab (for TAP1, Santa Cruz Biotechnology, Santa Cruz, CA) or mouse antiserum (for TAP2, generated in our laboratory [36]).
gondii P-gp minigene mouse antiserum, 1∶250 dilution.
