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In contrast to mouse antisera against cyclic peptides, rabbit antibodies against the linear HV2BD peptide, AbHV2BD linear, were bactericidal against OpaB variants with a bactericidal50 concentration of 40.4 µg/mL.
In studies with mouse antisera against cyclic peptides, mice were inoculated with a 20 µl suspension containing 6×103 CFU that were preincubated with AbHV2BD cyclic, AbSV cyclic, or NMS (final dilution of antiserum or NMS, 1∶30) (n = 10 11 mice per group).
Bacterial suspensions were diluted 1∶10 and pre-incubated for 5 min with mouse antisera against HV2 or SV cyclic peptides (test) or NMS (negative control) (final dilutions 1∶30 and 1∶100), or with 0.25 µg/mL or 2.5 µg/mL of AbHV2BD linear (test) or AbHV2C linear (negative control).
Mouse antisera against actin I, PPLP6, PK2, and CLK4 were generated for this study (see below).
In our experiments, epitope peptide pr4 showed strong reactivity with mouse antisera against DENV1-4 but not with anti-JEV mice sera.
Using mouse antisera against antigens present in FBJ cells the Gross (G) or wild type specificity of FBJ tumours was demonstrated by cross reactivity with antigens expressed on normal AKR lymphoid tissues and leukaemias.
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Polyclonal guinea pig antisera against purified influenza A virus and mouse antisera prepared against Influenza A/H3N2 hemagglutinin (HA0) cleavage loop peptides, were used in confocal fluorescence microscopy to show specific staining of wild-type influenza H1N1 and H3N2 viruses in clinical specimens.
Two general types of antibodies were evaluated in this study, specifically affinity-purified rabbit polyclonal antibodies against linear peptides (Ablinear) and mouse antisera raised against cyclic peptides (Abcyclic).
Western blots were performed to examine the reaction of native ApxIII with mouse antisera raised against the Ap97 and ApxN vaccines.
Western blot analyses on T. vaginalis total protein extracts with the mouse antisera raised against one of the cytoplasmic located peptides (CT-1) recognized a consistent major protein with an apparent molecular mass of ~52 kDa for both isolate G3 and SS-22, while all presera did not detect any material (additional file 17, Figure S6).
As observed with wild type FA1090 bacteria, AbHV2BD linear but not the AbHV2BD cyclic or AbSV cyclic mouse antisera was bactericidal against OpaB variants of strain FA1090F62 por5–8 in the presence of NHS.
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