Extracts obtained from organs of mouse and chicken were screened using P 3HB-co-4HB) film.
Figures 6 and 7 show the lipase activities of supernatants from mouse and chicken organs using pNPL as substrate.
Pancreatic extracts from both mouse and chicken showed similar depolymerizing activity as the commercial lipases on the P 3HB-co-4HB) film.
Lipase from P. cepacia was used as positive control to compare the depolymerizing activity of the organ extracts from mouse and chicken.
Adding crocodilians to the comparison of mouse and chicken allows the inference of ancestral and derived expression patterns.
The mouse and chicken inner ear develop similarly both at the cellular and molecular level [43], [44].
Thus, lentivirus with the human IRBP promoter driving GFP, specifically results in expression of GFP in photoreceptors of human, mouse and chicken retinas.
We constructed a lentivirus expressing GFP from the human IRBP promoter, and tested the specificity using human, mouse and chicken retinal explants.
Specific iNOS oligonucleotide primers were designed around the conserved regions in sequences aligned from the human, mouse and chicken iNOS genes [18], [27], [31], [32].
Both phenotypes were seen for human, mouse and chicken orthologs, but the penetrance varied depending on the species of origin (Table 3).
For immunocytochemistry, we also used two antibodies prepared against the Prdm1 C-terminal amino acid sequence (KVKQETVEPMDP) that is identical in human, mouse, and chicken Prdm1.
