Sentence examples for mouse Tbp confirmed from inspiring English sources
Real-time quantitative RT PCR using primers specific for mouse Tbp confirmed the absence of mouse cells in human XenoCT320 colospheres, whereas this approach always showed the presence of mouse (stromal) cells in human XenoCT320 tumour tissue (rate<10%).
The DCt value of the sample is determined by subtracting the Ct value of the mouse TBP gene from the Ct value of the Total TBP gene.
Results, expressed as N-fold differences in specific mouse Tbp gene expression (using mouse Tbp primers) relative to the sum of the mouse and the human TBP gene expression (using 'Total- TBP' primers), termed N Mm-TBP, are determined by theformula: N Mm-TBP = 2DCtsample.
Mouse Tbp was used as an internal control for all qRT-PCR experiments (Mm00446971_m1; Life Technologies).
As for calculation of % of mouse cells, specific mouse Tbp gene expression and the expression of both the mouse and the human TBP genes were studied by real-time qRT-PCR using the mouse Tbp as target gene and the 'Total- TBP' as endogenous RNA control.
First, the proportion of mouse cells was estimated in a panel of 8 different PDX types, using a real-time qRT-PCR assay combining primers specific for mouse Tbp RNA and primers able to amplify a common sequence on both human and mouse TBP transcripts.
Specific mouse TBP gene expression and the expression of both the mouse and the human TBP genes were studied by real-time quantitative RT PCR (Lévy et al, 2004) to determine the quantity of mouse cells in human xenografts and colospheres.
Despite systematic sputum analysis (induced or spontaneous), TBP was confirmed only by bronchoscopy in 38 subjects (24% of TBP).
Genotypes of mice were confirmed by PCR.
mRNA levels were normalized to TATA binding protein (TBP) to confirm equal cDNA input, using the 2−ΔΔCt method for calculation of the relative expression values.
The tbpE gene was selected as a probe because the expression of this protein, but not of the other TBP, had been confirmed in our proteomics study [ 38].
