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59 out of the 98 known imprinted genes in mouse are in the mouse RefSeq database.
To identify the SNP positions in the mouse RefSeq database, we used the SNP genotype and information in the Perlegen mouse SNP database (http://mouse.perlegen.com).perlegen.com
We used a local version of the NCBI BLAST program (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi) to align the 32-bp reads to the mouse RefSeq database (http://www.ncbi.nlm.nih.gov/RefSeq/).nih.gov/RefSeq/
This tool was applied to the mouse RefSeq database.
An example is given for the mouse RefSeq database (Supplementary Material).
For a control comparison, we also extracted 3' untranslated regions (3' UTRs) from the human and mouse RefSeq database.
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We then removed redundant sequences and predicted ncRNAs with sequences similar or identical to coding genes annotated in the Mouse RefSeq mRNA database [ 19], RIKEN cDNA [ 7], Mouse Protein NR database [ 20], or coding genes in other organisms annotated in GenBank [ 21].
Because no hits were made in the Xin repeat region, BLASTP was also used to search the mouse RefSeq protein database with the Xin repeat region sequences, using no filter and expect value = 10.
Sequences generated from high-throughput sequencing were aligned to the mouse RefSeq mRNA database.
Solexa DGE reads were aligned to the mouse refseq mRNA database [ 78] and chemosensory and chemosensory-related genes were selected from the putative mouse orthologs.
The cDNA sequence of Psg22 was then identified via BLAST analysis of the mouse RefSeq RNA database using the GenBank partial sequence referenced in Beauchemin et al. [ 37].
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