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The phthalates had no apparent effects on AR expression in mouse MEFs, whereas the AR levels were reduced in iPSCs.
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Total RNA was extracted from mouse MEF cells (negative control) and mouse SSCs using Trizol (Invitrogen).
We next demonstrated that N-Myc and Twist-1 co-expression transforms early-passage mouse embryo fibroblasts (MEFs), whereas each oncogene separately fails to do so.
Unexpectedly, VAR F1L was unable to protect both wild-type and Bax−/− mouse embryonic fibroblasts (MEFs), whereas Bak−/− MEFs were efficiently protected from apoptosis in a manner similar to Bcl-2, suggesting that VAR F1L exclusively inhibits Bax-mediated apoptosis.
Blockade of SFK activation abrogated TNF-α-activated gene expression in Thy-1− MEFs, whereas restoration of SFK activation rescued the TNF-α response in Thy-1+ MEFs.
Surprisingly, PP2 (10 µM) rescued SFK activation in TNF-α stimulated Thy-1+ MEFs, whereas PP2 exhibited little effect on SFK phosphorylation in Thy-1− MEFs (Figure 3B).
Heterozygous (wt/ki) MEFs expressed more vinculin than wild type MEFs, whereas (ki/ki) cells expressed 40% of wild type and a similar amount of ΔEx20 vinculin.
Addition of QVD gave partial protection in both WT and Drp1 −/− MEFs, whereas the Mlkl −/− MEFs were completely protected.
They grew significantly faster than Mdr2 +/+ MEFs, whereas Mdr2 +/− cells exhibited an intermediate growth rate.
Rad54 KO mouse embryonic fibroblasts (MEFs) were not found to be more radiosensitive to γ-rays than WT MEF, whereas embryonic neural stem cells require Rad54 for survival after irradiation.
We reported previously that wild-type mouse embryo fibroblasts (MEFs) differentiate to white fat cells, whereas MEFs lacking a functional retinoblastoma gene (designated Rb−/− MEFs) differentiate to brown adipocytes [23].
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