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Taken together, these results, through the definition of a detailed list of Klf5 transcriptional targets in mouse ESCs, support the important and specific functional role of Klf5 in the maintenance of the undifferentiated ESC phenotype.
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Also, they reported that a modified alginate-based 3D strategy supported proliferation and differentiation of the mouse ESCs (mESCs) into pancreatic insulin-producing cells (Wang et al. 2009; Chayosumrit et al. 2010).
These data support previous reports that Oct4 positively regulates Rif1 expression in mouse ESCs.
The induced piPSCs are dome-shaped mouse embryonic stem cells (ESCs -like and display molESCs -likeperties of mouse ESCs.
This analysis indicated that S/G2-enriched fractions of mouse ESCs (F13) generated at least 5-fold more pluripotent hybrid colonies than G1-enriched fractions (F8) and supported the idea that ESCs at later stages of cell cycle have a more potent reprogramming capacity.
Nuclear lysates were collected from v6.5 mouse ESCs or HCT116 cells following a revised Dignam protocol53.
Mouse ESCs were cultured on 0.1% gelatin-coated dishes in 5% CO2 at 37 °C.
Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines.
EB5 mouse ESCs (RIKEN BioResource Center) were maintained in mES medium for self-renewal.
Given the functional impact of BRD9 loss or inhibition on ESC pluripotency, we sought to further characterize BRD9's association with BAF complexes in mouse ESCs.
In mouse ESCs, both paralogs are expressed, which likely explains why genetic deletion of GLTSCR1 in ESCs had no adverse effects on pluripotency22.
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CEO of Professional Science Editing for Scientists @ prosciediting.com