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Mouse ESCs display similarities with ICM cells, cells of the epiblast of early post-implantation embryos and with primordial germ cells, and like ICM cells, they are heterogeneous in their pattern of protein and gene expression [7], [8].
Here we show that mouse ESCs display stress-related cellular phenotypes in response to androgens during early phase of differentiation.
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The induced piPSCs are dome-shaped mouse embryonic stem cells (ESCs -like and display molESCs -likeperties of mouse ESCs.
Furthermore, TET1-null mouse ESCs remain undifferentiated and express pluripotency factors, but again display skewed differentiation [ 112].
Here, we combine flow cytometry and a quantitative live single-cell imaging approach to demonstrate how the controlled differentiation of mouse ESCs towards a primitive streak fate in culture results in cells displaying many of the characteristics observed during early mouse development including transient brachyury expression, EMT and increased motility.
Nuclear lysates were collected from v6.5 mouse ESCs or HCT116 cells following a revised Dignam protocol53.
Mouse ESCs were cultured on 0.1% gelatin-coated dishes in 5% CO2 at 37 °C.
Mouse ESCs carrying translocated chromosomes can be isolated and expanded to establish stable cell lines.
EB5 mouse ESCs (RIKEN BioResource Center) were maintained in mES medium for self-renewal.
Given the functional impact of BRD9 loss or inhibition on ESC pluripotency, we sought to further characterize BRD9's association with BAF complexes in mouse ESCs.
In mouse ESCs, both paralogs are expressed, which likely explains why genetic deletion of GLTSCR1 in ESCs had no adverse effects on pluripotency22.
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