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After rinsing, the slides were counterstained with DAPI (Vectashield mounting, Vector Laboratories, Orton Southgate, Peterborough, UK) and coverslipped.
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Stained sections were mounted (Vector) and analyzed on a Leica confocal microscope.
Slides were mounted (Vector Labs, UK) and imaged with a confocal or bright-field microscope.
The sections were counterstained with propidium iodide (PI) for 5 minutes and mounted in vector shield (Vector laboratories).
After washed, samples were covered with VECTASHIELD mounting medium (Vector) and imaged in Leica SP5 confocal.
The sections were subsequently washed with PBS and mounted with Vectashield mounting medium (Vector laboratories, Inc. Burlingame, CA, USA).
Slides were mounted with mounting media (Vector Labs, USA).
Slides were mounted with anti-fading Vectashield mounting medium (Vector laboratories, Burlingame, CA).
After hybridization, the sections were mounted with mounting medium (Vector, USA).
Stained specimens were mounted in Vecta-shield mounting medium (Vector Labs).
Fluorescent preparations used to visualize gross synaptic morphology were mounted in Vectashield mounting medium (Vector laboratories).
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