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After mounting the specimen in Vectashield, a confocal stack of the brain was recorded using a ZEISS LSM confocal microscope.
Enclosing and mounting the specimen within the glass tube avoids contamination and satisfies the requirements of the SLOT setup for mechanical stability and optical transparency.
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After mounting, the specimens were analyzed under light microscopy.
Axial rotation was tested by mounting the specimens in the centre of the material-testing machine, so that the axis of torsion lies midway between the centre and the posterior edge of the vertebral body in the sagittal plane.
The first step was to mount the specimen and the simplest possible well-mount in distilled water was used.
After the sample was mounted, the specimen under test was pulled perpendicular to the wafer substrate until some part of the structure under ruptures.
She didn't mount the specimens in the conventional way.
After cell membrane perforation, we mounted the specimens with Vectashield® including DAPI for staining of cell nuclei.
After washing five times for 10 min each at room temperature, we mounted the specimens between two coverslips with Vectashield (Vector Laboratories, Burlingame, CA).
The proximal ends of the tibia and fibula were cylindrically embedded in Polymethylmethacrylate (Beracryl, Suter Kunststoffe AG, Fraubrunnen, Switzerland) in order to mount the specimens to the OR table.
This development occurred during the time it took to laser manipulate each sequence of embryos, including rinses to remove the fluorescent probe and re-mounting the specimens for imaging.
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