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Final washing and mounting steps remained the same as for single stainings.
After fixation, washing and mounting steps, fluorescent images of the slides were obtained using a confocal microscope (Zeiss LSM 510 META).
Cells were then denatured in 2 × SSC and 50% formamide at 80°C for 40 min. DNA FISH hybridizations were carried out with labelled DNA (10 ng/μl) and mouse Cot1 DNA (100 ng/μl) (Invitrogen) in 2 × SSC, 50% formamide and 12% dextran sulfate overnight at 37°C, followed by the same washing and mounting steps as described for RNA FISH.
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"You will mount first, yes?" Stathis is addressing Joe, who hops happily on to the mounting step as Hippo, a merry-looking, round-bellied ginger pony, is led out of the stable.
Paraffin sections were deparaffinized, and were rinsed in Fontana silver solution at room temperature away from light for 18 48 hours, and placed in 0.2% gold chloride solution, in 5% Na2S2O3 solution, and counterstained by Van Gieson solution, followed by dehydrated, transparented, and mounted steps.
It professes to start with something simple, exact, and invariable, and from this point to mount step by step till it comes to Man himself ; but indeed it starts with Man.
She snapped a picture with her cellphone, mounted two steps, stepped through a nonexistent front door and walked slowly across the floor.
Crowds are mounting the steps to mass.
Mounting the steps toward the view of ground zero will have a processional significance".
There have been unfortunate images of Trump in tennis shorts or mounting the steps to Air Force One.
One last time the faithful rose, mounting the steps and flocking into the pews of Lincoln Congregational Temple United Church of Christ, one of Washington's most historically significant African American churches.
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