Exact(1)
"You will mount first, yes?" Stathis is addressing Joe, who hops happily on to the mounting step as Hippo, a merry-looking, round-bellied ginger pony, is led out of the stable.
Similar(59)
Final washing and mounting steps remained the same as for single stainings.
After fixation, washing and mounting steps, fluorescent images of the slides were obtained using a confocal microscope (Zeiss LSM 510 META).
Cells were then denatured in 2 × SSC and 50% formamide at 80°C for 40 min. DNA FISH hybridizations were carried out with labelled DNA (10 ng/μl) and mouse Cot1 DNA (100 ng/μl) (Invitrogen) in 2 × SSC, 50% formamide and 12% dextran sulfate overnight at 37°C, followed by the same washing and mounting steps as described for RNA FISH.
Paraffin sections were deparaffinized, and were rinsed in Fontana silver solution at room temperature away from light for 18 48 hours, and placed in 0.2% gold chloride solution, in 5% Na2S2O3 solution, and counterstained by Van Gieson solution, followed by dehydrated, transparented, and mounted steps.
She snapped a picture with her cellphone, mounted two steps, stepped through a nonexistent front door and walked slowly across the floor.
Our study group investigated PCa detection rates for RTE with regard to tumour size and tumour volume in a whole-mount step section analysis [ 27].
These data are in line with a study by Junker et al., who also compared RTE findings with those of mpMRI in a whole-mount step section analysis [ 45].
The explanation might be the usage of an endfire probe for RTE in our population, which scans the prostate at different angles compared to the whole-mount step sections or to mpMRI.
Studies comparing preoperative elastograms with whole-mount step sections after RPE showed a sensitivity of 50 87%, a specificity of 72 92% for correct PCa localisation, and an overall PPV of 67 88% and a NPV of 44 83% [ 33– 33].
Sumura et al. used whole-mount step sections as the reference standard and reported a superiority of RTE detection rates to those of MRI (74.1% versus 47.4%) and nearly equal detection rates for RTE alone on both the anterior side (75.0%) and the posterior side (73.7%) of the prostate [ 44].
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