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His assistant Dorothy Thursby-Pelham (who would go on to become "England's first female sea-going fisheries scientist") produced a series of beautiful pencil sketches of the intact emperors before Assheton sliced up two of the specimens, mounting sections onto some 800 slides.
Prior to mounting, sections were counterstained with methyl green.
Before mounting sections in AquaMount (DAKO), they were once washed in PBS for 10 min at room temperature.
The method which is illustrated in Figure 1 has also the advantage of minimizing deformations resulting from mounting sections on slides, such as nonoptimal orientation of opercular or intrasulcal cortex, and facilitates comparisons between stainings and section levels with minimal bias since the morphological insula undergoes limited deformation.
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The plane sections remain plane after deformation (Navier-Bernoulli hypothesis); The distribution of deformations in the mounting section is linear.
Since 2009, police dogs have been cut by more than 200 and five forces have disbanded their mounted sections.
Dehydrate and mount sections for imaging.
FluorSave (CalBiochem) was used to mount sections.
The mounted sections were dehydrated and coverslipped as described above.
The mounted sections were left to air-dry for 7 days.
The mounted sections were subjected to H&E and immunochemical staining.
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