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Mounting observations indicate that type I interferons are implicated in the pathogenesis of several diseases, with autoimmune characteristics including SLE, primary Sjögren's syndrome, dermatomyositis, scleroderma and insulin-dependent diabetes mellitus [ 24].
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Observations lasted until mounting was observed or for a maximum of 10 min. Pairs were subsequently left together for another 24 72 h.
Some embryos were dehydrated with graded ethanol series and cleared with a 1 benzyl alcohol: 2 benzyl benzoate solution for whole-mount observation.
Finally, the sections were dehydrated, counterstained and mounted for observation.
Sections were then washed and mounted for observation under a scanning confocal microscope (TCS SP2 Leica).
Finally, the sections were counterstained by Meyer's Hematoxylin and mounted for observation.
Finally, slides were incubated with 3,3′-diaminobenzidine (DAB, Sigma-Aldrich Co). and H2O2, counterstained, dehydrated, and mounted for observation.
Then the cells were washed three times with PBS, and the plate was mounted for observation and imaging with a Leica TCS SP2 laser confocal scanning microscope (Wetzlar, Germany).
The signal was amplified by avidin-biotin complex formation and developed with diaminobenzidine followed by counterstaining with hematoxylin, after which the sections were dehydrated in alcohol and xylene, and mounted for observation.
After 10 min contact time, worms were washed with M9 plus 1 mM levamisole (600 × g, 2 min), transferred to 2% agarose (w/v in M9) pads and mounted for observation under fluorescent microscopy (Texas red filters).
Before mounting for microscopic observation, slides were rinsed twice for 5 min in PBS.
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