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Mounted samples (Vectashield mounting medium, from Vector Laboratories Inc., Burlingame, U.K). were analyzed with Zeiss LSM 510 Meta coupled to the confocal inverted Zeiss microscope (Axiovert 200 M; lasers: HeNe 633 nm, HeNe 543 nm, and Ar 488 nm, Zeiss, Feldbach, Switzerland).
Enhanced chemiluminescent reagent (ECL) was from Amersham (Freiburg, Germany) and Vectashield/DAPI mounting medium from Alexis (Grünberg, Germany).
After briefly washing the slides in PBS, the slides were mounted in fluorescence mounting medium from DAKO.
Slides were then mounted with DAPI containing mounting medium from Vector Laboratories.
MMP-7 and uPA were from Chemicon International, Harrow, UK; MMP-1 and mouse IgG1 from R&D Systems, Abingdon, UK; mouse anti-human pan cytokeratin from Sigma-Aldridge, Poole, UK; rabbit anti-human pan cytokeratin from Biogenesis Ltd., Poole, UK; rabbit anti mouse biotinylated antibody, swine anti-rabbit TRITC and aqueous mounting medium from DAKO Ltd., Cambridge, UK.
Chromosomes were counterstained in DAPI and mounted in VECTASHIELD® Mounting Medium from Vector Laboratories Inc.
Similar(53)
VectorMount mounting medium for immunofluorescence (IF) was purchased from Vector Laboratories (Burlingame, CA).
Mounting medium for fluorescence with DAPI was obtained from Vector Laboratories, Inc. (Burlingame, CA, USA).
Antifade® mounting medium was from Biomeda Corp. Alexa® Fluor 594 human transferrin was from Molecular Probes™.
Vectashield mounting medium was from Vector Laboratories, Burlingame, CA. Tissue-Tec OCT compound was purchased from Sakura Finetek Europe BV, Zoeterwoude, The Netherlands.
Vectashield mounting medium was from Vector Laboratories, Inc. (Burlingame, CA).
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