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Following 4′,6-diamidino-2-phenylindole counterstaining and mounting, images were taken using a × 63 oil-immersion lens objective with an LSM780 confocal microscope (Carl Zeiss, Oberkochen, Germany).
This can making mounting images an easier process.
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One wing from each fly was removed for slide mounting, image capture and analysis.
Collection consists of nineteenth- and early twentieth-century photographs: 9,000 individual, mounted images and about 150 albums containing approximately 4,000 images.
The mounted images, which vary from about 200 x 150mm to 450 x 300mm in size, are all numbered in the same hand in pencil, and it is probable that the volume was part reference and part sample book.
The collection, Codex MS 1413, was originally three volumes of mounted images.
This freeware program will allow you to create virtual disc drives and mount images to them.
Slides were mounted, imaged, and analyzed as described above.
In all, 15 20 wings from independent females were mounted, imaged, and measured using ImageJ.
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