For whole-mount detection of pERK, embryos were fixed with 4% paraformaldehyde (PFA) in PBS overnight at 4°C.
Whole-mount detection of Edu+ cells showed a widespread distribution in the brain of young (5 7 weeks old) subjects 4 h after injection (Fig. 1A E).
Vaginal swabs were used to prepare a wet mount (detection of trichomonads and clue cells, and after KOH addition yeasts and amine smell), a Gram stain for Nugent scoring (done centrally at the Institute of Tropical Medicine in Antwerp), and to inoculate Trichomonas vaginalis cultures.
Cells were fixed with 4% paraformaldehyde in PBS for 60 min and then mounted for detection by confocal microscopy.
Negative whole-mount detections were also verified after histological sections.
The steps for labeling of fixed cells for fluorescence microscopy are (1) sample preparation methods for using QDs, (2) mounting media, (3) detection with a fluorescence microscope, and (4) imaging QDs with a laser confocal microscope.
The proposed system fuses visual detections from wall mounted cameras and detections from a mobile robot in a centralized manner–employing a "tracking-by-detection" approach within a Particle Filtering strategy.
The procedures for administrating the primary antibodies (galectin-1 versus H-Ras or galectin-1 versus K-Ras), PLA probes, hybridization, ligation, amplification, detection, and mounting followed the manufacturer's recommended protocol.
Following detection and mounting of slides with antifade Prolong GOLD without DAPI (Invitrogen), signals were identified in BrdU incorporated nuclei as replicated doublets (D), or un-replicated singlet's (S), using a DMRXA epifluorescence microscope, DFC340 FX camera and CW4000 Karyo software (Leica).
A custom made forward detection mount with an attached photomultiplier tube (PMT) (Hamamatsu, H9305-04), and a band pass filter (transmittance = 475 - 485 nm) was employed to detect the SHG signal.
