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Fixed brains were sectioned and mounted using an identical method to that described above.
The phone and optical components were mounted using an optical rail system, and laid out as in Figure 1a.
After several washes in PBS, sections were incubated for 50 min in blocking solution containing a fluorescein-conjugated goat anti-mouse antibody (dilution 1∶100) then rinsed in PBS and mounted using an aqueous preparation (Fluoroprep, BioMérieux).
Finally, the slides were mounted using an aqueous mounting medium.
Samples were mounted using an aqueous mounting medium.
The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), and the slides were mounted using an antifade reagent.
Similar(45)
was performed according to the manufacturer's protocol and mounted using a xylene-based mounting solution (Pertex, Surgipath).
The slides were counterstained with hematoxylin and dehydrated in graded alcohols, air-dried and mounted using a resin-based mounting medium (Immunotech) under coverslips.
Slides were mounted using a Vectashield mounting medium H-10000), Vector LaBurlingame InCaliforniangame, California, USA).
Here's a crash course: A pivot door is mounted using a set of pins in lieu of a traditional hinge.
Manual z-adjustment is provided by a New Focus 9063-COM gothic-arch transtageon stage mounted using a New Focus 9063-A angle bracket.
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