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Slide mounted sections were incubated in PBS for 5 min, and blocked for 60 min in PBS containing 10% donkey serum and 0.25% Triton X-100.
The mounted sections were treated with 0.1% Triton X-100 for 5 minutes, blocked with 5% non-immune goat serum, and probed with anti-p-Smad2 anti-p-Smad2 anti-p-Smad2abbit antibody.
For Prussian blue staining, slide mounted sections were deparaffinized, rehydrated, and reacted for 30 minutes in 2% potassium ferrocyanide and 3.7% hydrochloric acid to visualize ferric iron particles by Prussian blue.
The mounted sections were dehydrated and coverslipped as described above.
The mounted sections were left to air-dry for 7 days.
The mounted sections were subjected to H&E and immunochemical staining.
Similar(29)
Digestion of mounted sections was carried out with 50 μg/ml proteinase K at 55°C for 120 min.
Isolated RNA that met the quantity and quality specifications from each of the slide mounted sections was tested twice in separate assay runs.
Labeling of wholemounts and slide mounted sections was visualized using an Olympus BX51 fluorescent microscope equipped with appropriate filters and an Optronics digital camera.
Prior to mounting, sections were counterstained with methyl green.
The plane sections remain plane after deformation (Navier-Bernoulli hypothesis); The distribution of deformations in the mounting section is linear.
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