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The aluminum pin with mounted sample sits inside a stainless steel holder.
The field-cycling method is based on precise mechanical positioning of the NMR probe with the mounted sample in the inhomogeneous fringe field of the spectrometer magnet.
After filtering a concentrated cell suspension, the filter was gently rinsed with buffer, carefully cut (1 cm ×1 cm), attached to a steel sample puck (Veeco Metrology Group) and the mounted sample was transferred into the AFM liquid cell while avoiding dewetting.
In short, the trace element data are the result of an average of four measurements per mounted sample (table 2), taken with a laser beam diameter of 55 µm at 70% of the laser energy (0.2 mJ) at a frequency of 15 Hz.
A scheme of the mounted sample is presented in Figure 1.
Monochromatic X-rays were gathered using a capillary condenser prior to passing through a pinhole and illuminating the mounted sample.
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The mounted samples were immersed in juice medium to obtain a constant potential, which is referred as the Open Circuit Potential.
Alloy composition and phases were identified by SEM EDS (semi-quantitative) on mounted samples in order to observe and analyse details of the microstructure.
In addition, corrosion layers were observed in mounted samples using a polarized light microscope BK-POL/BKPOLR BK-POL/BKPOLRtured by Alltion Comodel in dark field, at Faculty of Conservation, Art University of Isfahan.
Whole mounted samples, taken from different developmental stages, were fixed and stained with fluorophore-labeled WGA lectin and phalloidin for the analysis of tegument and muscles, respectively.
The latter is demonstrated by having mounted samples of a-C H Si O on the exterior of the Interna-C H Si Oace Station via the Matexteriornternatiofal Space StheInternational Space Station
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