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Wild-type mouse cerebella were dissected at different postnatal ages, fixed in alcohol/acetic acid (95% /5% or paraformaldehyde (4%) as indicated, embedded in paraffin, sliced, and mounted on silanized glass (Silane, Sigma Co., St . Louis MO, USA).
Six micrometer thick sections were mounted on silanized glass slides and processed using the Apoptag Plus Peroxidase Apoptosis Detection Kit (Chemicon, Temecula, CA) according to manufacturers' protocol.
Histological sections of 4 µm were mounted on silanized slides and allowed to dry for 1 h at room temperature (RT), followed by 1 h incubation in an oven at 60°C.
Sections were mounted on silanized slides and allowed to dry overnight at 37°C.
Sections were cut and mounted on silanized slides by American Histolabs (Gaithersburg, MD).
Sections 4 μm thick were cut from paraffin-embedded tissue blocks and mounted on silanized slides.
Similar(43)
It was mounted on a plastic frame.
Tissues sections (5 µm) were mounted onto silanized glass microscope slides and dried for 7 days at 37°C prior to use.
Eight-micrometer (8 μm -thick paraffin sections were cut and mounted onto silanized slides.
Sections were cut at 4 μm thicknesses, mounted onto silanized slides, and left to dry overnight at 37°C.
Briefly, fixed explants were embedded in paraffin, sectioned at approximately 4-μm thickness and mounted onto silanized slides.
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