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Coverslips were mounted on microscopy slides (Fisherbrand, 12-550-003, TherMowiol®er Scientific) using mountingmediumng medium (Calbiochem, 475904, Millipore Co., Billerica, MA, USA) supplemented with DAPI (50 μg/mL).
Cells were mounted on microscopy slides.
The tissue was frozen in OCT®, then eight micron thin cryo-sections were cut and mounted on microscopy slides (Gold Seal Rite-On Micro Slides, Portsmouth, NH) and epithelial cells were isolated as described previously [26].
Coverslips were mounted on microscopy slides.
Coverslips were washed with PBS and mounted on microscopy glass slides with 90% glycerol in PBS.
The coverslips were mounted on microscopy slides with Fluoromont G (Immunkemi, Stockholm, Sweden).
Similar(51)
Following immunostainings, slices and sections, still including culturing membranes, were washed with KPBS + Triton X100 and KPBS before mounting on microscopy slides using DABCO fixation.
Roots were incubated at room temperature for at least 24 h in Alexa Fluor staining solution, containing 137 mM NaCl, 2.7 mM KCl, 12 mM Na2HPO4/KH2PO4 and 0.01% [w/v] Alexa Fluor® 488 conjugate of wheat germ agglutinin (Invitrogen), and then washed five times prior mounting on microscopy slides.
Sections of 10 µm thickness were mounted on Superfrost microscopy slides (Thermo Fisher Scientific Inc., Gothenburg, Sweden) and incubated overnight at 4°C with anti-FosB antibody (1 300, catalog number H-237; Santa Cruz Biotechnology, Santa Cruz, CA, USA).
The worms were stained with 0.125% thioflavin S (Sigma) in 50% ethanol for 2 min, destained for 2 min in 50% ethanol, washed with PBS and mounted on slides for microscopy.
Stained cells were washed with PBS and deionised water and mounted on slides for microscopy.
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