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The slices were then fixed for one hour in 4% paraformaldehyde, washed with PBS and mounted on microscope slides.
Coverslips were mounted on microscope glass slides using Fluro-Gel with DAPI (Electron Microscopy Science) followed by visualization using confocal microscopy (Leica SP5 confocal with resonant scanner).
Finally, the cells were washed twice with cold PBS and mounted on microscope slides using buffered mounting medium.
Finally, the cells were mounted on microscope slides using a buffered mounting medium after washed twice with cold PBS.
The stained root samples were mounted on microscope slides in polyvinyl lacto glycerol (PVLG) and examined for AMF colonization under light microscope.
Processed slices were mounted on microscope slides using Mowiol.
Microscope cover glasses were then mounted on microscope slides.
After several washes with PBS, coverslips were mounted on microscope slides with Mowiol (Calbiochem).
Dissections were made using tungsten needles, and appendages were mounted on microscope slides.
Sections were washed (3 times, 20 min), mounted on microscope slides, and photographed.
Slices were then incubated with Alexa 488-phalloidin (Invitrogen) in the dark for 15 minutes and mounted on microscope slides.
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CEO of Professional Science Editing for Scientists @ prosciediting.com