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Following PBS rinses, the sections were mounted on coated slides (Fisher Super Frost Plus), air dried, dehydrated through a graded series of alcohol, cleared with Citrasolv (Fisher Scientific), and coverslipped with DPX (Sigma).
Sections were mounted on coated slides, coversliped and dried before analysis.
Sections were mounted on coated slides, dehydrated, cover slipped, viewed and photographed using Zeiss and Leitz microscope and a Nikon digital camera, immunoreactive elements plotted onto the atlas depictions [35], and images edited using photoshop (vCS; Adobe systems).
Sections were rinsed with PB 3×10 min and incubated for 2 hrs at room temperature with one of the following secondary fluorescent antibodies: anti mouse or anti rabbit (Invitrogen, Carlsbad, CA), rinsed with PB 3×10 min, mounted on coated slides and coversliped.
Sections (3 μm thick) were mounted on coated slides (BDH).
Free-floating sections were mounted on coated slides.
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The sections were mounted on Formvar-coated plastic specimen holders.
Sections were mounted on Vectabond coated slides (Vector Labs, CA), dehydrated and covered using Depex.
Here, sections were washed, mounted on gelatine coated slides and coverslipped with Entellan (MercK, Darmstadt, Germany).
Sections (7 μM) were mounted on silan coated glass slides (Menzel gläser).
Following dewaxing, tissue sections mounted on sialane coated slides were subjected to the following procedures.
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