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Slides were subjected to graded ethanol rinses, cleared in Xylene and mounted in Pertex.
Sections were then dehydrated, cleared in xylene, and mounted in Pertex Mounting Medium.
Immunoreactivity was detected using the chromagen, 3,3'-diaminobenzidine (ImmPACT DAB, Vector) followed by counterstaining of sections with Harris's haematoxylin (Pioneer Research Chemicals, Colchester, UK) and mounted in Pertex (Cell-path, Hemel Hempstead, UK).
After dehydration, slides were mounted in Pertex (Histolab, Gothenburg, Sweden).
The slides were mounted in Pertex (Leica Microsystems, Kista, Sweden).
The sections were finally mounted in Pertex mounting medium.
Similar(43)
Nuclei were counterstained with haematoxylin before mounting in Pertex (Histolab, Göteborg, Sweeden).
Visualization was performed using 3,3′-diaminobenzidine tetrahydrochloride (DAB, DAKO) and sections were counterstained with haematoxylin before mounting in Pertex mounting medium (CellPath plc, Hemel Hempstead, UK).
DAB (1 50) was used to visualise staining, and Mayer's haematoxylin (BDH Laboratories, Poole, UK) was incubated for 30 seconds as a counterstain prior to mounting in Pertex mounting media.
Visualization was performed using 3,3-diaminobenzidine tetrahydrochloride (Dako) and sections were counterstained with haematoxylin, dehydrated in graded alcohols and immersed in xylene before mounting in Pertex mounting medium (CellPath plc, Hemel Hempstead, UK).
Four others were mounted in 1934.
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