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Distal colonic tissue specimens were mounted in modified Ussing chambers and transepithelial resistance was recorded [17].
For in vitro studies, the intestinal segments mounted in modified Ussing chambers were exposed to different concentration of ETX after a brief period of stabilization and monitored during 60 minutes.
Each intestinal sheet (1 cm in length) without any visible Peyer's patch with a support of nylon gauze were mounted in modified Ussing chambers (Warner instruments, CA) with an exposed surface area of 0.25 cm2 and maintained at 37°C by water-jacketed reservoirs.
Jejunal specimens were mounted in modified Ussing chambers to carry out impedance measurements as described previously [ 44].
Fluorescent whole-mount in situ specimens were mounted in modified Sca leA2 containing 20% glycerol, 2.5% DABCO and 4 M urea (Hama et al., 2011).
After 2 hr, the animals were rinsed with an excess of 1× Montjuic salts, fixed in cold 4% paraformaldehyde (in 1× Montjuic salts), mounted in modified ScaleA2 and photographed using a Zeiss LSM-510 VIS confocal microscope.
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After electrophoresis, the cover was removed and either the PDMS chip or the PMMA cover was mounted in a modified MALDI ion source for analysis.
Rats were subsequently mounted in a modified stereotaxic frame.
Biopsies were mounted in miniaturized modified Ussing air-suction chambers.
The DD, CC, CAD, and CD tissue specimens were mounted in a modified Ussing chamber [ 29] and bathed with 3.5 ml of Parson buffer solution on both sides of the intestinal wall.
After rapid excision, the heart was mounted in a modified Langendorff system and then perfused with the nominally Ca2 + -free Tytode solution (NaCl 137 mM、KCl 5.4 mM、HEPES 100 mM、Glucose 0.33 mM、HEPES 10 mM、Glucose 10 mM, and PH7.35 with NaOH) for 5 min at room temperature (20-25)°C.
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